Journal: Cancers
Article Title: Circulating Tumour Cell Associated MicroRNA Profiles Change during Chemoradiation and Are Predictive of Response in Locally Advanced Rectal Cancer
doi: 10.3390/cancers15164184
Figure Lengend Snippet: ( A ). Enumeration of circulating tumour cells (CTCs) from a patient isolated using the Isoflux TM instrument. The upper panel shows a CTC (green arrow) and lymphocytes (red arrow) and the lower panel is an enlargement of the area indicated. The identified CTC is DAPI-positive, pan-CK positive, and CD45 negative. Immunomagnetic beads can be seen in the background. Scale bar: 50 µm upper panel, 20 µm lower panel. ( B ). Heatmap representing the CTCs identified from 52 patients at baseline, during treatment and post-treatment. These time-points have 1, 4, and 11 samples missing respectively (represented by the white boxes). Each column on the X-axis represents a patient. Green represents low numbers with orange to red colour gradient representing increasing numbers of CTCs.
Article Snippet: We enumerated CTCs during chemoradiation at these three time-points, using the Isoflux TM (Fluxion Biosciences Inc., Alameda, CA, USA) CTC Isolation and detection platform.
Techniques: Isolation